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Journal: Animals : an Open Access Journal from MDPI
Article Title: PGE 2 Modulates Uterine Luminal Fluid Composition and Endometrial Function in Dairy Heifers During Diestrus
doi: 10.3390/ani16071037
Figure Lengend Snippet: The effect of PGE 2 treatment on IFNT receptor expression in bEEC. ( A ) PPI network analysis of DAPs involved in cellular response to type I interferon. ( B ) GSEA analysis of cellular response to the type I interferon pathway. ( C ) GSEA analysis of the JAK-STAT signaling pathway. ( D ) Analysis of IFNAR1 expression in bEECs after PGE 2 treatment with different concentrations (10 −8 , 10 −7 , and 10 −6 M). ( E ) Analysis of IFNAR2 expression in bEECs after PGE 2 treatment with different concentrations (10 −8 , 10 −7 , and 10 −6 M). ( F ) Representative images of PTGER2, IFNAR1, STAT1, and p-STAT1 protein expression in bEECs after interference with PTGER2. ( G – I ) Analyses of PTGER2, IFNAR1, and p-STAT1 protein expression in bEECs after interference with PTGER2, respectively. ( J ) Representative images of PTGER4, IFNAR1, STAT1, and p-STAT1 protein expression in bEECs after interference with PTGER4. ( K – M ) Analyses of PTGER4, IFNAR1, and p-STAT1 protein expression in bEECs after interference with PTGER4, respectively. ( N – P ) Analysis of ISG15 , MX2, and GRP expression in bEECs after interference with PTGER4, respectively. “**” represents p < 0.01; “*” represents p < 0.05; “ns” represents p > 0.05.
Article Snippet: The membranes were then incubated overnight at 4 °C with the following primary antibodies: anti-OPN antibody (1:1000, 22952-1-AP, Proteintech, Wuhan, China), anti-FN1 antibody (1:1000, 22952-1-AP, Proteintech), anti-ZO-1 antibody (1:1000, BY9025, Abways, Shanghai, China), anti-CDH1 antibody (1:1000, 3195S, Cell Signaling Technology, Danvers, MA, USA), anti-β-catenin antibody (1:500, sc-53484, Santa Cruz, CA, USA), anti-PTGER2 antibody (1:1000, ab167171, Abcam, Cambridge, UK), anti-STAT1 antibody (1:1000, 14994, Cell Signaling Technology), anti-p-STAT1 antibody (1:1000, 7649, Cell Signaling Technology),
Techniques: Expressing